Amylin/DAP Kit ELISA

N° du produit ABIN4881297

Kit ELISA Amylin/DAP Humain, Souris et Rat, Colorimetric test pour la quantification de Humain, Souris et Rat Amylin/DAP.

Aperçu rapide pour Amylin/DAP Kit ELISA (ABIN4881297)

Antigène: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Amylin/DAP Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Amylin (IAPP) EIA Kit optimized for serum and cell culture medium. Competition-based ELISA

Analytical Method: Quantitative

Specificité: Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Sensibilité: 0.62 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated Amylin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Autre désignation: Amylin

Sujet:

Gene Symbols: IAPP

Background: Amylin, or Islet Amyloid Polypeptide (IAPP), is a 37-residue peptide hormone. It is cosecreted with insulin from the pancreatic beta-cells. Amylin plays a role in glycemic regulation by slowing gastric emptying and promoting satiety, thereby preventing post-prandial spikes in blood glucose levels. IAPP is processed from an 89-residue coding sequence. Proislet Amyloid Polypeptide is produced in the pancreatic beta cells (beta-cells) as a 67 amino acid, 7404 Dalton pro-peptide and undergoes post-translational modifications including protease cleavage to produce amylin.

Insulin and IAPP are regulated by similar factors since they share a common regulatory promoter motif. The IAPP promoter is also activated by stimuli which do not affect insulin, such as tumor necrosis factor alpha and fatty acids. One of the defining features of Type 2 diabetes is insulin resistance. This is a condition wherein the body is unable to utilize insulin effectively, resulting in increased insulin production, since proinsulin and proIAPP are cosecreted, this results in an increase in the production of proIAPP as well.

Amylin"s metabolic function is well-characterized as an inhibitor of the appearance of nutrient in the plasma. It thus functions as a synergistic partner to insulin, with which it is cosecreted from pancreatic beta cells in response to meals. The overall effect is to slow the rate of appearance of glucose in the blood after eating, this is accomplished via coordinate slowing down gastric emptying, inhibition of digestive secretion [gastric acid, pancreatic enzymes, and bile ejection, and a resulting reduction in food intake. Appearance of new glucose in the blood is reduced by inhibiting secretion of the gluconeogenic hormone glucagon. These actions, which are mostly carried out via a glucose-sensitive part of the brain stem, the area postrema, may be over-ridden during hypoglycemia. They collectively reduce the total insulin demand. < h4>Product Features< div class="desc-content">

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Protein Name (Synonyms): Islet amyloid polypeptide (Amylin) (Diabetes-associated peptide) (DAP) (Insulinoma amyloid peptide)

ID gène: 3375

UniProt: P10997

Pathways: Hormone Activity, Feeding Behaviour