SULT1C2 Kit ELISA

N° du produit ABIN4884689

Détail du produit SULT1C2 Kit ELISA

Antigène: SULT1C2 (Sulfotransferase Family, Cytosolic, 1C, Member 2 (SULT1C2))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human SULT1C2 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SULT1C2 (Sulfotransferase Family, Cytosolic, 1C, Member 2 (SULT1C2))

Autre désignation: SULT1C2 (SULT1C2 Produits)

Synonymes: ST1C1 Kit ELISA, ST1C2 Kit ELISA, SULT1C1 Kit ELISA, humSULTC2 Kit ELISA, 1810008N17Rik Kit ELISA, Sult1c1 Kit ELISA, st1c1 Kit ELISA, st1c2 Kit ELISA, sult1c1 Kit ELISA, MGC88979 Kit ELISA, SULT1C2 Kit ELISA, Sult1c2 Kit ELISA, sulfotransferase family 1C member 2 Kit ELISA, sulfotransferase family, cytosolic, 1C, member 2 Kit ELISA, sulfotransferase family 1C member 2 S homeolog Kit ELISA, sulfotransferase 1C2 Kit ELISA, SULT1C2 Kit ELISA, Sult1c2 Kit ELISA, sult1c2 Kit ELISA, sult1c2.S Kit ELISA, LOC100060302 Kit ELISA, LOC100729651 Kit ELISA, LOC100623541 Kit ELISA

ID gène: 6819

UniProt: O00338