BMP6 Protéine
Aperçu rapide pour BMP6 Protéine (ABIN7865993)
Antigène
Voir toutes BMP6 ProtéinesType de proteíne
Activité biologique
Origine
Source
Pureté
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Fonction
- Human BMP6 Protein Recombinant
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Attributs du produit
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Protein Size: Native humanBMP-6 isgenerated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately17 kDa. Recombinant BMP-6 is a disulfide-linked homodimeric protein consisting of two 140 amino acid residue subunits,and migrates as an approximately34 kDa protein under non-reducing conditions and as a 17 kDa protein under reducing conditions in SDS-PAGE.
Sequence Position: Optimized DNA sequence encoding Human Bone Morphogenetic protein-6 mature chain was expressed in Escherichia Coli.
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Stérilité
- 0.2 μm filtered
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niveau d'endotoxine
- Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).
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Biological Activity Comment
- The ED(50) was determined by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic and was found to be in the range of0.1-0.5 ug/ml.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
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Buffer
- Recombinant Bone morphogenetic Protein-6was lyophilized from a 0.2 μm filtered solution in 2.5 % glycine, 0.5 % sucrose, 0.01 % Tween80, 5 mM Glutamic acid, pH 4.5.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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Date de péremption
- 24 months
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- BMP6 (Bone Morphogenetic Protein 6 (BMP6))
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Autre désignation
- BMP6
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Poids moléculaire
- 34 kDa
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Pathways
- Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process
Antigène
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