Choline Oxidase anticorps
Aperçu rapide pour Choline Oxidase anticorps (ABIN116681)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
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Purification
- Prepared from monospecific antiserum by a delipidation and defibrination.
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Immunogène
- Choline Oxidase [Alcaligenes species].
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anti-Choline Oxidase antibody
Reactivité: Alcaligenes WB, ELISA, IP Hôte: Chèvre Polyclonal unconjugated
anti-Choline Oxidase antibody (HRP)Reactivité: Alcaligenes WB, ELISA, IP Hôte: Chèvre Polyclonal HRP
anti-Choline Oxidase antibody (Biotin)Reactivité: Alcaligenes WB, ELISA, IP Hôte: Chèvre Polyclonal Biotin
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Indications d'application
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Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Choline Oxidase[Alcaligenes species] in a standard sandwich ELISA using Peroxidase conjugated AffinityPurified anti-Goat IgG [H&L] (Rabbit) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 4,000 to 1: 32,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Restore with 2.0 mL of deionized water (or equivalent).
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Concentration
- 90.0 mg/mL (by Refractometry)
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Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.
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- Choline Oxidase
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Sujet
- Choline Oxidase catalyzes the oxidation of choline and betaine aldehyde to betaine with concomitant consumption of oxygen and production of hydrogen peroxide.
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ID gène
- 512
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UniProt
- P16101
Antigène
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