Cet anticorps anti-Invertase Polyclonal Chèvre (ABIN117966) détecte spécifiquement Invertase dans WB, EIA et IP.
L’anticorps est réactif avec des échantillons de Candida sp..
Aperçu rapide pour Invertase anticorps (ABIN117966)
Antigène
Invertase
Reactivité
Candida sp.
Hôte
Chèvre
Clonalité
Polyclonal
Conjugué
Cet anticorp Invertase est non-conjugé
Application
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)
Specificité
This product was prepared from monospecific antiserum. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Invertase [Candida]. Cross reactivity against Invertase from other sources is unknown.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Invertase [Candida] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 10,000 to 1: 65,000 of the reconstitutionconcentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 2.0 mL of deionized water (or equivalent).
Concentration
90.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Antigène
Invertase
Sujet
Invertase hydrolyses the terminal non reducing beta D fructofuranoside residues in beta D fructofuranosides. It is the enzyme that bees use to convert nectar into honey. Industrial and confectionary applications use yeast invertase to split sucrose into fructose and glucose and also to improve the shelf life of food products.Synonyms: Beta-fructofuranosidase, INV1, Saccharase