Aperçu rapide pour R-Phycoerythrin anticorps (Texas Red (TR)) (ABIN401163)
Antigène
R-Phycoerythrin
(Phycoerythrin, R-)
Hôte
Chèvre
Clonalité
Polyclonal
Conjugué
Cet anticorp R-Phycoerythrin est conjugé à/à la Texas Red (TR)
Application
Flow Cytometry (FACS), Immunofluorescence (IF)
Attributs du produit
Absorption / Emission: 596 nm / 620 nm Molar Ratio: 2.5 Moles Texas Red(TM) per mole of Goat IgG
Purification
Immunoaffinity Chromatography using a R-Phycoerythrin coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Immunogène
Highly purified R-Phycoerythrin from the seaweed gracila
This product is designed for immunofluorescence microscopy, fluorescence based plateassays (FLISA) and fluorescent western blotting. This product is also suitable for multiplexanalysis, including multicolor imaging, utilizing various commercial platforms. Immunofluorescence: 1/500-1/2500. Flow Cytometry: 1/2000-1/10000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent) For extended storage, restore with 50 % glycerol
Concentration
1.0 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 10 mg/mL BSA (IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Antigène
R-Phycoerythrin
(Phycoerythrin, R-)
Autre désignation
R-Phycoerythrin
Classe de substances
Chemical
Sujet
Phycoerythrin is one of a series of fluorescent pigments known as phycobiliproteins, which are produced by red and blue green algae. It occurs in more than one form, and has found application in immunology and diagnostic medicine. B and R Phycoerythrins provide superior labeling compared to fluorescein and rhodamine, and are used for labeling antibodies, usually monoclonals. These dyes may also be coupled to enzymes and other proteins, nucleic acids, polypeptide hormones, drugs, etc. Since phycoerythrins absorb light maximally between 450 and 650nm they fill the need for an intense fluorescent dye in the longer wavelengths of the visible spectrum, thereby avoiding interference from naturally fluorescing biological substances. R Phycoerythrin (240 kDa) is a labile molecule that may dissociate into components upon exposure to reducing or denaturing agents.Synonyms: rpeB