APOBEC3A anticorps (Internal Region)
Aperçu rapide pour APOBEC3A anticorps (Internal Region) (ABIN238645)
Antigène
Voir toutes APOBEC3A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- Phorbolin 1 / APOBEC3A
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Séquence
- TSNFNNGIGR HKTY
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Réactivité croisée
- Humain
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence C-TSNFNNGIGRHKTY, from the internal region of the protein sequence according to NP_663745.1.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of distinct parts of the nucleus in lymphoid cells. Recommended concentration: 3-6 μg/mL.
Western Blot: Approx. 26-28 kDa band observed in Human Spleen, Tonsil and Peripheral Blood Monocytes (PBM) lysates (calculated MW of 23.0 kDa according to Human NP_663745.1). This molecular weight is routinely observed by other sources. Recommended concent
Peptide ELISA: antibody detection limit dilution 1:8000.
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Commentaires
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Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and A549 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration:
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- APOBEC3A (Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A))
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Autre désignation
- APOBEC3A
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Sujet
- APOBEC3A, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A, ARP3, PHRBN, bK150C2.1, phorbolin 1
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ID gène
- 200315
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NCBI Accession
- NP_663745
Antigène
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