ASS1 anticorps (Internal Region)
Aperçu rapide pour ASS1 anticorps (Internal Region) (ABIN374809)
Antigène
Voir toutes ASS1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- Internal Region
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Séquence
- C-ENPKNQAPPG LYTKTQD
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Specificité
- This antibody detects ASS1.
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human, Cow. -
Purification
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
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Immunogène
- Peptide from the internal region of the protein sequence according to NP_000041.2, NP_446464.1. Genename: ASS1
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Indications d'application
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Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: Approx 45 kDa band observed in Human Kidney lysates (calculated MW of46.5 kDa according to NP_000041.2 and NP_446464.1). Recommended concentration: 0.01-0.03 μg/mL. Immunofluorescence: A customer has reported this antibody works well in IF. Immunoprecipitation: This antibody has been successfully used in immunoprecipitationassays (Corbin et al. , 2008).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- ASS1 (Argininosuccinate Synthase 1 (ASS1))
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Autre désignation
- ASS1
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Sujet
- ASS1 belongs to the argininosuccinate synthase family, type 1 subfamily. It catalyzes the penultimate step of the arginine biosynthetic pathway. Defects in ASS1 are the cause of citrullinemia type 1 (CTLN1) [MIM:215700].Synonyms: Argininosuccinate synthase, Citrulline-aspartate ligase
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Poids moléculaire
- 46.5 kDa (NP_000041.2 and NP_446464.1)
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ID gène
- 445, 9606
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UniProt
- P00966
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Pathways
- Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin
Antigène
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