GATA1 anticorps (Internal Region)
Aperçu rapide pour GATA1 anticorps (Internal Region) (ABIN185449)
Antigène
Voir toutes GATA1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- GATA1
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Séquence
- DAEAYRHSPV FQ
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Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence C-DAEAYRHSPVFQ, from the internal region of the protein sequence according to NP_002040.1.
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Isotype
- IgG
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Indications d'application
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Western Blot: Approx 50-55 kDa band observed in nuclear lysates of cell line K562 calculated MW of 42.8 kDa according to NP_002040.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL. Primary incubation 1
Peptide ELISA: antibody detection limit dilution 1:16000.
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Commentaires
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Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and NIH3T3 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 1
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
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Autre désignation
- GATA1
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Sujet
- GATA1, GATA binding protein 1 (globin transcription factor 1), HGNC:4170, ERYF1, GF1, NFE1, GATA binding protein 1, GATA-binding protein 1 (globin transcription factor 1), erythrold transcription factor 1, globin transcription factor 1, transcription fact
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ID gène
- 2623
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NCBI Accession
- NP_002040
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Pathways
- Cellular Response to Molecule of Bacterial Origin
Antigène
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