Aperçu rapide pour Chèvre anti-Lapin IgA, IgG, IgM (Fab Region), (Fc Region) Anticorps (HRP) (ABIN568664)
Antigène
IgA, IgG, IgM
Clonalité
Polyclonal
Conjugué
HRP
Application
Immunofluorescence (IF), Western Blotting (WB), Immunodiffusion (ID), Dot Blot (DB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
Isotype
IgG
Épitope
Fab Region, Fc Region
Reactivité
Lapin
Hôte
Chèvre
Attributs du produit
Molar Ratio: Peroxidase/IgG: aprox. 1.7
Purification
Hyperimmune antisera with strong precipitating activity are selected for fractionation by Salt-Precipitation and purifaction of the IgG fraction by DEAE Chromatography
Immunogène
Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore by adding 1.0 mL sterile distilled water.
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.
Antigène
IgA, IgG, IgM
Classe de substances
Antibody
Sujet
The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by immuno immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in rabbit serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties.Synonyms: Rabbit IgA, Rabbit IgG, Rabbit IgM