SEMA5A anticorps (Internal Region)
Aperçu rapide pour SEMA5A anticorps (Internal Region) (ABIN185680)
Antigène
Voir toutes SEMA5A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- Internal Region
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Fonction
- Semaphorin 5A
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Séquence
- DEATKKACYS KGKS
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Specificité
- The immunizing peptide corresponds to a part of the extracellular domain.
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Réactivité croisée
- Boeuf (Vache), Chien, Humain, Souris, Rat
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence DEATKKACYSKGKS, from the internal region of the protein sequence according to NP_003957.1.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration: 4-6 μg/mL.
Western Blot: Preliminary experiments gave an approx 90 kDa in Mouse Brain lysates and approx 25 kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03 μg/mL antibody staining. Please note that currently we cannot find an explanati
Peptide ELISA: antibody detection limit dilution 1:128000.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- SEMA5A (Sema Domain, Seven thrombospondin Repeats (Type 1 and Type 1-Like), Transmembrane Domain (TM) and Short Cytoplasmic Domain, (Semaphorin) 5A (SEMA5A))
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Autre désignation
- SEMA5A
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Sujet
- SEMA5A, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A, HGNC:10736, SEMAF, semF, sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane
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ID gène
- 9037, 20356, 310207
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NCBI Accession
- NP_003957
Antigène
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