HA-Tag anticorps
(7 références)Aperçu rapide pour HA-Tag anticorps (ABIN387711)
Antigène
Hôte
Clonalité
Conjugué
Application
Clone
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Séquence
- YPYDVPDYA
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Specificité
- THETM HA Tag Antibody, mAb, Mouse (A01244) recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins.
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Attributs du produit
- Anti-HA-tag mAb (ABIN387711) is a highly sensitive monoclonal antibody applicable to HA-tagged fusion protein detection. It belongs to our elite antibody collection. HA, or human influenza hemagglutinin, is a surface glycoprotein required for human influenza virus infectivity. The HA tag does not appear to interfere with the bioactivity or the biodistribution of recombinant proteins. Anti-HA-tag mAb (Antibodies-Online, ABIN387711) is a high-affinity monoclonal antibody that can be used to detect HA-tagged proteins. This product detects HA tags derived from an HA molecule corresponding to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors.
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Purification
- Protein A affinity column
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Immunogène
- A KLH coupled synthetic peptide (YPYDVPDYA)
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Isotype
- IgG1
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anti-HA-Tag antibody
WB, IF, ChIP Hôte: Lapin Polyclonal RB0411 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP, IF, FACS Hôte: Souris Monoclonal 18B11H6 unconjugated
anti-HA-Tag antibodyWB, ELISA, IP Hôte: Lapin Polyclonal unconjugated
anti-HA-Tag antibodyWB, ELISA, IF, ChIP Hôte: Lapin Polyclonal unconjugated
anti-HA-Tag antibodyReactivité: Humain WB Hôte: Souris Monoclonal 12CA5 unconjugated
anti-HA-Tag (AA 98-106) antibodyAnimal-Free Reactivité: Tag WB, ELISA, IF Hôte: Lapin Monoclonal AB158-1-D11 unconjugated Recombinant Antibody
anti-HA-Tag antibody (HRP)WB, ELISA, IP Hôte: Souris Monoclonal 5E11D8 HRP
anti-HA-Tag (AA 98-106) antibodyAnimal-Free Reactivité: Tag WB, ELISA, IF Hôte: Souris Monoclonal AB158-1-D11 unconjugated Recombinant Antibody
anti-HA-Tag antibodyReactivité: Tag WB, ELISA Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mL
Immunoprecipitation: 1 µg/mL
Other applications: user-optimized -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- PBS, pH 7.4, containing 0.02 % Sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing cycles.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below.
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: "APOBEC3-mediated restriction of RNA virus replication." dans: Scientific reports, Vol. 8, Issue 1, pp. 5960, (2019) (PubMed).
: "Novel coronavirus-like particles targeting cells lining the respiratory tract." dans: PLoS ONE, Vol. 13, Issue 9, pp. e0203489, (2019) (PubMed).
: "Functional analysis of bovine Nramp1 and production of transgenic cloned embryos in vitro." dans: Zygote (Cambridge, England), Vol. 23, Issue 1, pp. 83-92, (2015) (PubMed).
: "Dominant drug targets suppress the emergence of antiviral resistance." dans: eLife, Vol. 3, (2014) (PubMed).
: "Evaluating the mutagenic activity of targeted endonucleases containing a Sharkey FokI cleavage domain variant in zebrafish." dans: Zebrafish, Vol. 10, Issue 3, pp. 353-64, (2013) (PubMed).
: "Phosphatidylinositol-4-phosphate 5-kinase isoforms exhibit acyl chain selectivity for both substrate and lipid activator." dans: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 35953-63, (2012) (PubMed).
: "Dissecting the conserved NPxxY motif of the M3 muscarinic acetylcholine receptor: critical role of Asp-7.49 for receptor signaling and multiprotein complex formation." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 28, Issue 5, pp. 1009-22, (2011) (PubMed).
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- HA-Tag
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Autre désignation
- HA Tag
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Classe de substances
- Tag
Antigène
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