LDHD Kit ELISA
Aperçu rapide pour LDHD Kit ELISA (ABIN454979)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.
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Analytical Method
- Quantitative
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Specificité
- This assay recognizes recombinant and natural Mouse D-LDH.
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Réactivité croisée (Details)
- No significant cross-reactivity or interference was observed.
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Sensibilité
- The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
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Attributs du produit
- Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4
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Lactate Dehydrogenase D (LDHD) ELISA Kit
LDHD Reactivité: Souris Colorimetric Sandwich ELISA 4.69 mU/mL - 300 mU/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD Reactivité: Humain Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
Lactate Dehydrogenase D (LDHD) ELISA KitLDHD Reactivité: Humain Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Protocole
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- LDHD (Lactate Dehydrogenase D (LDHD))
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Autre désignation
- Ldhd
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Pathways
- Ribonucleoside Biosynthetic Process
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