Phosphotyrosine anticorps (HRP)
Aperçu rapide pour Phosphotyrosine anticorps (HRP) (ABIN135060)
Antigène
Hôte
Clonalité
Conjugué
Application
Clone
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Attributs du produit
- The antibody detects: Phosphorylated tyrosine and phenylphosphate
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Purification
- Purified
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Isotype
- IgG2b
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anti-Phosphotyrosine antibody
Reactivité: Différentes espèces WB, FACS, ICC Hôte: Souris Monoclonal P-Tyr-01 unconjugated
anti-Phosphotyrosine antibodyReactivité: Différentes espèces WB, IHC (p) Hôte: Souris Monoclonal 3F6 unconjugated
anti-Phosphotyrosine antibody (PerCP)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 PerCP
anti-Phosphotyrosine antibody (HRP)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 HRP
anti-Phosphotyrosine antibody (Atto 594)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 Atto 594
anti-Phosphotyrosine antibody (Biotin)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 Biotin
anti-Phosphotyrosine antibody (FITC)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 FITC
anti-Phosphotyrosine antibody (APC)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 APC
anti-Phosphotyrosine antibody (PE)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 PE
anti-Phosphotyrosine antibody (Atto 390)WB, IP, IF, IHC, ICC Hôte: Souris Monoclonal G104 Atto 390
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Indications d'application
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Working Dilution:
ELISA: 1:1,000-1:4,000
Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded. -
Volume d'échantillon
- 1 mL
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Product is supplied as 1.0 mL of stock solution in 50 % glycerol / 50 % PBS, pH 7.4.
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Conseil sur la manipulation
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Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed. -
Stock
- 4 °C
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- Phosphotyrosine
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Classe de substances
- Amino Acid
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Sujet
- Protein tyrosine residues are phosphorylated as a result of intracellular protein kinase activation (e.g. via growth factors) during normal growth and development, and in oncogenesis. The most abundant population of target proteins for tyrosine phosphorylation are cell surface glycoproteins. Antibody to phosphotyrosine enables the detection, isolation and characterization of proteins containing phosphotyrosine. PY20 also prevents internalization of activated receptors, e.g. EGFr, when microinjected into cells. The affinity of PY20 for phosphotyrosine is approximately 10-6 to 10-7 M. PY20 binding to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by phosphoserine, phosphothreonine or free phosphate.
Antigène
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