Cité dans 1 publication.
Cet anticorps anti-Ig Light Chains Monoclonal Souris (Clone IVA285-1) (ABIN94401) détecte spécifiquement Ig Light Chains dans FACS, IHC (fro) et WB.
L’anticorps est réactif avec des échantillons de Boeuf (Vache) et Mouton.
The antibody IVA285-1 reacts with bovine Ig (light chains). Ig light chains (kappa and lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Western blotting: Recommended dilution: 0,5-1 μg/mL, overnight at 4 °C. Sample preparation: bovine serum, dilution 1:50 in Laemmli reducing buffer, boiled in water bath for 3 min. Reducing conditions, 12 % separating gel. The antibody strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow cytometry: Recommended dilution: 1-4 μg/mL
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze
Stock
4 °C
Stockage commentaire
Store at 2-8°C. Do not freeze.
Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." dans: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, (2006) (PubMed).