Fibroblasts anticorps
Aperçu rapide pour Fibroblasts anticorps (ABIN472810)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Recognizes human fibroblasts in tissue as well as cultured fibroblasts. On skin sections the antibody reacts with stratum basal epidermal cells. The antigen appears to be a marker for mesodermally dervied (mesenchymal) human connective tissue and tumors derived from such tissue (e.g. fibrosarcomas) {Haynes et al, 1984}. Useful for the assessment of thymic epithelial culture contamination with fibroblasts, and the identification of cells of mesodermal origin in the fetus.
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Purification
- Ion exchange chromatography
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Immunogène
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Whole human thymic stoma cells.
Type of Immunogen: Cells -
Isotype
- IgG1
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Indications d'application
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Approved: Flo (1:100), IF (1:100 - 1:200)
Usage: Suitable for use in Immunofluorescence, FACS, and the assessment of thymic epithelial culture contamination with fibroblasts and the identification of cells of mesodermal origin in the fetus. Immunofluorescence: 1:100-1:200 (acetone fixed tissue). FACS: 1:100 (fresh or acetone fixed tissue, do not use trypsin to prepare adherent cell lines, use a non-enzymatic detachment procedure. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, 10 mM sodium azide, 1 mg/mL BSA.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- avoid freeze thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Fibroblasts
Antigène
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