Souris anti-Humain IgA Anticorps
Aperçu rapide pour Souris anti-Humain IgA Anticorps (ABIN457921)
Antigène
Clonalité
Application
Clone
Isotype
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Reactivité
- Humain
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Hôte
- Souris
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Specificité
- The reactivity of the antiserum is restricted to determinants on the C H 2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, indirect binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglu-tination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). Monoclonal mouse antiserum to human IgA, isotype specific
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Purification
- Delipidated, heat inactivated, lyophilized, stable whole ascites
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Immunogène
- Highly purified monoclonal IgA isolated from pooled human serum
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Mouse anti-Human IgA (Heavy Chain) antibody
ELISA, WB, IHC (fro), IP Monoclonal Mc24-2E11 IgG1 unconjugated
Goat anti-Human IgA antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Goat anti-Human IgA (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgA (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, WB Polyclonal IgG Alkaline Phosphatase (AP)
Rabbit anti-Human IgA (Chain alpha), (Heavy Chain) antibodyELISA, IHC, WB unconjugated
Goat anti-Human IgA (Heavy Chain) antibody (Biotin)ELISA, WB, FACS Polyclonal IgG Biotin
Goat anti-Human IgA (Heavy Chain) antibody (FITC)ELISA, FACS Polyclonal IgG FITC
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Indications d'application
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To identify the presence of IgA in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining of cytoplasmic IgA, haemagglutination and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1000 upwards. These data should be interpreted as general recommendations only. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
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Concentration
- 1 mg/mL
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Buffer
- No foreign proteins added.
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Agent conservateur
- Without preservative
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Conseil sur la manipulation
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- IgA
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Classe de substances
- Antibody
Antigène
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