LAT2 anticorps (AA 196-212)
Aperçu rapide pour LAT2 anticorps (AA 196-212) (ABIN120728)
Antigène
Voir toutes LAT2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 196-212
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Specificité
- The antibody recognizes the defined epitope (aa 196-212) of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
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Purification
- Protein A affinity chromatography
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Pureté
- > 95 % (by SDS-PAGE)
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Immunogène
- Peptide corresponding to aa 196-212 of human NTAL coupled to KLH
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Isotype
- IgG1
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anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody
LAT2 Reactivité: Humain, Souris WB, IP, ICC, ICFC Hôte: Souris Monoclonal NAP-07 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-209) antibodyLAT2 Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 Reactivité: Souris, Rat IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE)LAT2 Reactivité: Humain, Souris ICFC Hôte: Souris Monoclonal NAP-07 PE
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 32-192) antibodyLAT2 Reactivité: Rat WB Hôte: Lapin Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 196-212) antibodyLAT2 Reactivité: Humain WB, IHC, IP, IHC (p), Inhibition Hôte: Souris Monoclonal NAP-03 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB14275 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 124-203), (Cytoplasmic Domain), (N-Term) antibodyLAT2 Reactivité: Souris WB Hôte: Lapin Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB15935 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 Reactivité: Humain WB, FACS Hôte: Souris Monoclonal 24GB14270 unconjugated
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Indications d'application
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Immunoprecipitation. Western Blot: Recommended dilution: 1-2 mg/mL, incubation 60 min. Positive control: RAMOS human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mLcold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non- reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 12 % separating SDS-PAGE gel.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
Date de péremption
- 12 months
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- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
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Autre désignation
- LAT2 / WBSCR15
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Sujet
- NTAL (Non-T cell Activation Linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
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ID gène
- 7462
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UniProt
- Q9GZY6
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Pathways
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
Antigène
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