COL18A1 anticorps
Aperçu rapide pour COL18A1 anticorps (ABIN307188)
Antigène
Voir toutes COL18A1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Recognizes mouse endostatin, Mr 22kD, an unidentified protein is also weakly detected, Mr 54kD. Detects human protein in IHC-FFPE. It is predicted that the antibody will also detect the rat protein. Cross-reactivity is predicted based on sequence homology. The antibody has not been specifically tested with rat samples at this time.
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Purification
- Thiophilic adsorption
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Immunogène
- Full-length recombinant murine endostatin.
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Isotype
- IgG
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anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1581-1680) antibody
COL18A1 Reactivité: Humain, Souris, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1154-1335) antibodyCOL18A1 Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 237-263), (N-Term) antibodyCOL18A1 Reactivité: Humain, Souris WB, FACS Hôte: Lapin Polyclonal RB22312 unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1311-1339) antibodyCOL18A1 Reactivité: Humain, Souris WB, IHC (p) Hôte: Lapin Polyclonal RB22701 unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) antibodyCOL18A1 Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal KT60 unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (C-Term) antibodyCOL18A1 Reactivité: Humain, Souris WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1600-1754) antibodyCOL18A1 Reactivité: Humain ELISA, IF Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 801-850) antibodyCOL18A1 Reactivité: Humain, Souris IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (AA 1314-1343), (Chain alpha 1), (Middle Region) antibodyCOL18A1 Reactivité: Humain, Souris WB, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated
anti-Collagen, Type XVIII, alpha 1 (COL18A1) (C-Term) antibodyCOL18A1 Reactivité: Humain, Rat, Chien WB, IHC Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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Approved: IHC, IHC-P (1:100), WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100.Western Blot: 1:500-1:2000. Detects endostatin in mouse liver cell lysates to which 50 ng of recombinant human endostatin was added. Mouse liver cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with LS-B2229. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. -
Commentaires
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 50 mM sodium phosphate, pH 8.0, 0.05 % sodium azide, before the addition of glycerol to 30 %. Sourced in Ascites.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeat freeze-thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))
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Autre désignation
- COL18A1 / Endostatin
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Classe de substances
- Chemical
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Sujet
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Name/Gene ID: COL18A1
Family: Collagen
Synonyms: COL18A1, Collagen, type XVIII, alpha 1, Collagen alpha-1(XVIII) chain, Endostatin, KNO, KNO1, KS, Multi-functional protein MFP, Antiangiogenic agent, Collagen alpha 1(xviii), Knobloch syndrome, type 1 -
ID gène
- 80781
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UniProt
- P39060
Antigène
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