Collagen Type I anticorps (Biotin)
Aperçu rapide pour Collagen Type I anticorps (Biotin) (ABIN2619406)
Antigène
Voir toutes Collagen Type I (COL1) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- Collagen Type I from adult human knee cartilage and bovine nasal cartilage.
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Purification
- Affinity purified
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Immunogène
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Type of Immunogen: Native protein
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Indications d'application
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Approved: ELISA (1:3000 - 1:6000), IHC, IHC-P (10 μg/mL), IP, WB (1:3000 - 1:6000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- deionized water
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Concentration
- Lot specific
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Buffer
- 0.1 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 10 mg/mL BSA, 0.01 % Sodium Azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- 4°C or -20°C, Avoid freeze-thaw cycles.
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- Collagen Type I (COL1) (Collagen, Type I (COL1))
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Autre désignation
- Collagen I
Antigène
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