MYL12A anticorps (AA 10-35)
(3 références)
Aperçu rapide pour MYL12A anticorps (AA 10-35) (ABIN129691)
Antigène
Voir toutes MYL12A AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
-
-
Épitope
- AA 10-35
-
N° du produit (Fournisseur)
- 600-401-938
-
Fournisseur
- Rockland
-
Fonction
- Myosin Antibody
-
Réactivité croisée (Details)
- This affinity purified antibody is directed against the regulatory light chain of smooth and non-muscle myosin. The antibody detects both unphosphorylated and monophosphorylated forms of the protein.
-
Attributs du produit
- Synonyms: rabbit anti-Myosin antibody, Myosin regulatory light polypeptide 12A, Myosin regulatory light chain 12A, Myosin regulatory light chain MRLC3
-
Purification
- affinity purified antibody
-
Stérilité
- Sterile filtered
-
Immunogène
-
Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids aa 10-35 of human myosin light chain protein.
Immunogen Type: Conjugated Peptide
-
Isotype
- IgG
-
-
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (N-Term) antibody
MYL12A Reactivité: Humain, Souris WB, IHC, ELISA, IP Hôte: Lapin Polyclonal unconjugated
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (AA 92-171) antibodyMYL12A Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (pSer19), (pSer20) antibodyMYL12A Reactivité: Avian, Mammifères WB, IP, EIA, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (C-Term) antibodyMYL12A Reactivité: Humain WB, ELISA Hôte: Lapin Polyclonal unconjugated
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (AA 10-171) antibodyMYL12A Reactivité: Humain WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A) (N-Term) antibodyMYL12A Reactivité: Humain WB, IHC, ELISA, IP Hôte: Lapin Polyclonal unconjugated
-
-
Indications d'application
-
Immunohistochemistry Dilution: User Optimized
Application Note: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.
Western Blot Dilution: 1:500 - 1:2,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:70,000
Other: User Optimized
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 0.71 mg/mL
-
Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- 4 °C,-20 °C
-
Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
-
Date de péremption
- 12 months
-
-
-
: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." dans: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).
: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).
: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." dans: eLife, Vol. 4, pp. e08314, (2016) (PubMed).
-
-
- MYL12A (Myosin, Light Chain 12A, Regulatory, Non-Sarcomeric (MYL12A))
-
Autre désignation
- MYL12A
-
Sujet
- Background: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.
-
ID gène
- 10627
-
NCBI Accession
- NP_001289978
-
UniProt
- J3QRS3
Antigène
-
