Lipoprotein, beta 1 anticorps
L’anticorps anti-Lipoprotein, beta 1 Polyclonal Lapin est utilisé pour la détection de Lipoprotein, beta 1 dans des échantillons de Humain. Il a été validé pour IP.
N° du produit ABIN458082
287,13 €
Plus frais de livraison 40,00 € et TVA
1 mL
Destination:
France
Envoi sous 2 à 4 jours ouvrables
Aperçu rapide pour Lipoprotein, beta 1 anticorps (ABIN458082)
Antigène
Lipoprotein, beta 1
Reactivité
Humain
Hôte
Lapin
Clonalité
Polyclonal
Application
Immunoprecipitation (IP)
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Specificité
- Precipitating polyclonal Rabbit antiSerum to Human beta-1 lipoprotein (LDL).
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Réactivité croisée (Details)
- Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiSerum has not been tested in detail.
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Attributs du produit
- The defined antibody specificity is restricted to beta-1 lipoprotein as tested at the level of sensitivity of immunoprecipitation techniques. The antiserum does not react with any other protein component of human serum or plasma. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence transferrin in human serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.
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Immunogène
- Beta-1 lipoprotein has a molecular weight of 2,700,000. The protein is isolated from normal serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
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Indications d'application
- In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in 3 mm diameter centre well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
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Buffer
- Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
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Agent conservateur
- Without preservative
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Stock
- RT,4 °C,-20 °C
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Stockage commentaire
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.
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- Lipoprotein, beta 1
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Autre désignation
- beta-1 lipoproteins
Antigène
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