AGAP2 anticorps (AA 1-836)
Aperçu rapide pour AGAP2 anticorps (AA 1-836) (ABIN129537)
Antigène
Voir toutes AGAP2 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 1-836
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N° du produit (Fournisseur)
- 200-401-946
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Fournisseur
- Rockland
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Fonction
- AGAP2 Antibody
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Réactivité croisée (Details)
- This protein A purified antibody is directed against human AGAP2 protein.
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Attributs du produit
- Synonyms: rabbit anti-AGAP2 antibody, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 antibody, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 antibody, centaurin gamma1 antibody, Centg1 antibody
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Purification
- The product was purified from monospecific antiserum by protein A chromatography.
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Immunogène
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Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.
Immunogen Type: Recombinant Protein
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Isotype
- IgG
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anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (Internal Region) antibody
Verified AGAP2 Reactivité: Humain WB, ELISA, IHC Hôte: Chèvre Polyclonal unconjugated
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (AA 20-60) antibodyAGAP2 Reactivité: Humain, Singe WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (AA 450-500) antibodyAGAP2 Reactivité: Humain, Souris, Rat, Cheval, Singe WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (AA 850-877), (C-Term) antibodyAGAP2 Reactivité: Humain, Souris WB Hôte: Lapin Polyclonal RB19565 unconjugated
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (AA 719-768) antibodyAGAP2 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe, Roussette (Chauve-souris) WB Hôte: Lapin Polyclonal unconjugated
anti-ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2) (C-Term) antibodyAGAP2 Reactivité: Humain, Souris WB, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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Application Note: This protein A purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to AGAP2 protein by western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:5,000 - 1:20,000
ELISA Dilution: 1:40,000 - 1:200,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
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Concentration
- 2.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- AGAP2 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))
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Autre désignation
- AGAP2
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Sujet
- Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.
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ID gène
- 116986, 7661962
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UniProt
- Q99490
Antigène
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