Mouton anti-Lapin IgG (Heavy & Light Chain) Anticorps (TRITC)
Aperçu rapide pour Mouton anti-Lapin IgG (Heavy & Light Chain) Anticorps (TRITC) (ABIN619446)
Antigène
Clonalité
Conjugué
Application
Isotype
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Épitope
- Heavy & Light Chain
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Reactivité
- Lapin
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Hôte
- Mouton
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Fonction
- Sheep anti-Rabbit IgG (H&L), TRITC conjugated
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Purification
- Immunogen affinity purified
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Immunogène
- Purified rabbit IgG (H&L)
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Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
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Indications d'application
- The optimal working dilution should be determined by the investigator, Suggested starting dilution(s): 1 : 20-1 : 2000 for most applications
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 1,1 mL of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
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Buffer
- Immunogen affinity purified sheep IgG.
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Conseil sur la manipulation
- Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
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- IgG
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Classe de substances
- Antibody
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Sujet
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Background: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm.
Additional information: Purity of this preparation is > 95 % based on SDS-PAGE. Antibody concentration is 1.0 mg/mL (E 1 % at 280 nm = 13.0). Antibody is supplied in 10 mM sodium phosphate, 0.15 M sodium chloride, pH 7.2.1 % (w/v) B, Protease/IgG free. Contains 0.05 % (w/v) sodium azide as preservative of bacterial growth.Based on immunoelectrophoresis, this antibody reacts with: heavy chains on rabbit IgG, light chains on all rabbit immunoglobulins. Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins.
Antigène
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