Orf1 (N. Meningitidis) anticorps

N° du produit ABIN125757

Cité dans 1 publication. Cet anticorps anti-Orf1 (N. Meningitidis) Polyclonal Lapin (ABIN125757) détecte spécifiquement Orf1 (N. Meningitidis) dans WB. L’anticorps est réactif avec des échantillons de Neisseria meningitidis.

Aperçu rapide pour Orf1 (N. Meningitidis) anticorps (ABIN125757)

Antigène: Orf1 (N. Meningitidis)

Reactivité: Neisseria meningitidis

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB)

Détail du produit

Fonction: Anti-Orf1 (N. meningitidis) Purified

Réactivité croisée (Details): Neisseria meningitidis

Attributs du produit: The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Information d'application

Indications d'application: Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Prochazkova, Osicka, Linhartova, Halada, Sulc, Sebo: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." dans: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).

Détail du antigène

Antigène: Orf1 (N. Meningitidis)

Sujet: Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD

ID gène: 35