ATP6V0D2 anticorps (epsilon subunit)

N° du produit ABIN3197488

Il existe 3+ publications pour ce produit. L’anticorps anti-ATP6V0D2 Polyclonal Lapin est utilisé pour la détection de ATP6V0D2 dans des échantillons de Arabidopsis thaliana, Nicotiana tabacum, Tomato, Oryza sativa, Alfalfa, Zea mays, Vitis vinifera, Apple, Thellungiella, Orge, Chara australis, Chlamydomonas reinhardtii, Cucumis sativus, Fortunella margarita, Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Noccaea caerulescens, Petunia hybrida, Pineapple, Poplar (Populus), Pteris vittata (Chinese ladder brake), Salicornia bigelovii et Triticum aestivum. Il a été validé pour WB, IF et IHC.

Aperçu rapide pour ATP6V0D2 anticorps (epsilon subunit) (ABIN3197488)

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Reactivité: Arabidopsis thaliana, Nicotiana tabacum, Tomato, Oryza sativa, Alfalfa, Zea mays, Vitis vinifera, Apple, Thellungiella, Orge, Chara australis, Chlamydomonas reinhardtii, Cucumis sativus, Fortunella margarita , Lilium longiflorum (Trumpet lily), Mesembryanthemum crystallinum, Noccaea caerulescens, Petunia hybrida, Pineapple, Poplar (Populus), Pteris vittata (Chinese ladder brake), Salicornia bigelovii, Triticum aestivum

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATP6V0D2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Détail du produit anti-ATP6V0D2 anticorps

Épitope: epsilon subunit

Fonction: Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase

Réactivité croisée (Details): Ananas comosus, Arabidopsis thaliana, Cucumis sativus, Chara australis , Chlamydomonas reinhardtii, Fortunella margarita Swingle, Hordeum vulgare, Lycopersicum esculentum, Lilium longiflorum, Malus x domestica Borkh. c.v. Fuji, Medicago truncatula, Mesembryanthemum crystallinum, Nicotiana tabacum, Noccaea caerulescens, Oryza sativa, Petunia hybrida cv. Mitchell, Populus sp., Pteris vittata (fern), Salicornia bigelovii, Thellungiella sp., Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in: Avicennia sp., mangrove plants, Schizosaccharomyces pombe

Homologie: Brachypodium dystachyon,Capsella rubella,Chenopodium quinoa,Citrus clementina,Citrus unshiu,Citrus limon,Eucalypsus grandis,Glyxine max,Glycine soja,Lotus japonicus,Phaseolus sp.,Physcomitrium patens,Populus trichocarpa,Prunus persica,Ricinus communis,Riticum aestivum,Solanum lycopersicum,Solanum tuberosum,Sorghum bicolor Theobroma cacao,Vitis vinifera,Bull frog,Chicken,Bovine,Drosophila melanogaster,Human,Mouse,Rat

Purification: Serum

Immunogène: KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase including Arabidopsis thaliana UniProt: Q39258-1

Information d'application

Indications d'application: 1: 100 (IF), 1 : 50 (IHC), 1 : 2000-1 : 5000 (WB)

Commentaires:

V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.Immunostaining protocol using V-ATPase antibodies can be found here.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Conseil sur la manipulation: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock: -20 °C

Stockage commentaire: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Références pour anticorps anti-ATP6V0D2 (ABIN3197488)

Bigeard, Rayapuram, Bonhomme, Hirt, Pflieger: "Proteomic and phosphoproteomic analyses of chromatin-associated proteins from Arabidopsis thaliana." dans: Proteomics, Vol. 14, Issue 19, pp. 2141-55, (2014) (PubMed).

Schnell, Han, Miki, Johnson: "Soybean peroxidase propeptides are functional signal peptides and increase the yield of a foreign protein." dans: Plant cell reports, (2010) (PubMed).

Hinton, Sennoune, Bond, Fang, Reuveni, Sahagian, Jay, Martinez-Zaguilan, Forgac: "Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells." dans: The Journal of biological chemistry, Vol. 284, Issue 24, pp. 16400-8, (2009) (PubMed).

Détails sur ATP6V0D2

Antigène: ATP6V0D2 (ATPase, H+ Transporting, Lysosomal 38kDa, V0 Subunit D2 (ATP6V0D2))

Autre désignation: V-ATPase

Sujet:

Background: Plant vacuole V-ATPase is responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

Additional information: Cellular [compartment marker] of tonoplast membrane.This product can be sold containing ProClin if required.

Poids moléculaire: 26 | 31 kDa (Arabidopsis thaliana)

ID gène: 826716

UniProt: Q39258

Pathways: Transition Metal Ion Homeostasis, Proton Transport