Thrombospondin 1 anticorps

N° du produit ABIN305761

Cet anticorps Souris Monoclonal détecte spécifiquement Thrombospondin 1 dans WB, IHC et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Thrombospondin 1 anticorps (ABIN305761)

Antigène: Thrombospondin 1 (THBS1)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Thrombospondin 1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Thrombospondin 1 anticorps

Specificité: Designed for sensitive detection of TSP by Western Blot. It shows no cross-reactivity with fibronectin, fibrinogen and von Willebrand factor. Species cross-reactivity: human.

Purification: Protein G purified

Immunogène: Purified human TSP.

Type of Immunogen: Purified protein

Isotype: IgG1

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Reduced and non-reduced. 0.5-1.0 μg/mL for 2 hours at RT. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4.

Agent conservateur: Azide free

Conseil sur la manipulation: Aliquot to Avoid freeze/thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term 4°C (no longer than 1 week)
Long term -20°C
Aliquot to avoid freeze/thaw cycles.

Détails sur Thrombospondin 1

Antigène: Thrombospondin 1 (THBS1)

Autre désignation: Thrombospondin 1

Pathways: Autophagy