SMPD1 anticorps

N° du produit ABIN306944

Détail du produit anti-SMPD1 anticorps

Antigène: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMPD1 est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Humain

Attributs du produit: Acid Sphingomyelinase, aSMase, SMPD1, ASM, Sphingomyelin phosphodiesterase, ASM-1,Human acid sphingomyelinase (sphingomyelin phosphodiesterase, ASM) is the lysosomal enzyme responsible for the hydrolysis of sphingomyelin to ceramide and phosphocholine. Converts sphingomyelin to ceramide. aSM also has phospholipase C activities toward 1,2-diacylglycerol-phosphocholine and 1,2-diacylglycerol-phosphoglycerol. The enzyme is a membrane-associated glycoprotein with a pH optimum of about 4.5 and a subunit molecular mass of about 72 kDa. In addition AtoS M, two other sphingomyelinases have been identified in man, a Mg2+- dependent neutral sphingomyelinase found primarily in brain and a Zn2+-dependent acid sphingomyelinase found primarily in serum. Although it is likely that the acid and neutral sphingomyelinases are coded by different genes, the molecular genetic relationship of these three biochemically distinct sphingomyelinases has not been determined. Understanding the role of these sphingomyelinases in the hydrolysis of sphingomyelin to ceramide will be an important step in the understanding of ceramide as it is further hydrolyzed to sphingosine, a neutral phospholipid which has been implicated in the regulation of protein kinase C-mediated signal transduction. Inherited deficiencies of ASM have been reported in man, deficient ASM activity results in the two major subtypes of Niemann-Pick disease (NPD).

Purification: Ammonium Sulfate Precipitation

Immunogène: Rabbits were immunized with a synthetic peptide derived from human acid sphingomyelinase protein.

Information d'application

Indications d'application: Antibody can be used for Western blots (5-10 μg/mL) and ELISA. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur SMPD1

Antigène: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Autre désignation: Acid Sphingomyelinase (SMPD1 Produits)

Synonymes: anticorps ASM, anticorps ASMASE, anticorps NPD, anticorps A-SMase, anticorps Zn-SMase, anticorps aSMase, anticorps SMPD1, anticorps sphingomyelin phosphodiesterase 1, anticorps sphingomyelin phosphodiesterase 1, acid lysosomal, anticorps sphingomyelin phosphodiesterase, anticorps SMPD1, anticorps Smpd1, anticorps LOC5578088

UniProt: P17405