PRKG1 anticorps (AA 657-671)

N° du produit ABIN307178

L’anticorps anti-PRKG1 Polyclonal Lapin est utilisé pour la détection de PRKG1 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Porc, Poulet, Chien, Roussette (Chauve-souris), Cheval, Singe et Lapin. Il a été validé pour WB, IHC et IHC (p).

Aperçu rapide pour PRKG1 anticorps (AA 657-671) (ABIN307178)

Antigène: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Poulet, Chien, Roussette (Chauve-souris), Cheval, Singe, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PRKG1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PRKG1 anticorps

Épitope: AA 657-671

Specificité: Recognizes human PKG1 alpha at ~75kD.

Homologie: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Pufferfish (93%) Xenopus, Zebrafish (87%) Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Purification: Protein A purified

Immunogène: A 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Pufferfish (93%), Xenopus, Zebrafish (87%), Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (10 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 50 % glycerol, 0.09 % azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur PRKG1

Antigène: PRKG1 (Protein Kinase, CGMP-Dependent, Type I (PRKG1))

Autre désignation: PKG / PRKG1

Sujet: Name/Gene ID: PRKG1
Subfamily: CGMP
Family: Protein Kinase

Synonyms: PRKG1, CGKI, CGMP kinase I, CGMP protein kinase type I, CGK-I, CGK1, PKGI, PKG I, PRKGR1B, PRKG1B, PRKGR1A, PGK, PKG, CGK, CGK 1, CGKI-alpha, CGKI-BETA

ID gène: 5592

UniProt: Q13976