PEDF anticorps (AA 196-347)

N° du produit ABIN337197

Cet anticorps anti-PEDF Monoclonal Souris (ABIN337197) détecte spécifiquement PEDF dans WB, IHC, ELISA, ICC, IHC (p) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour PEDF anticorps (AA 196-347) (ABIN337197)

Antigène: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PEDF est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-PEDF anticorps

Épitope: AA 196-347

Specificité: Recognizes human GST-PEDF fusion protein with a MW of ~46kD.

Purification: Protein A purified

Immunogène: Recombinant human GST-PEDF fusion protein. The sequence corresponding to aa196-347 of human PEDF tagged with GST on the N-terminal was expressed in E. coli. GST-PEDF is about 46kD on SDS-PAGE.

Type of Immunogen: Recombinant protein

Isotype: IgG2a

Information d'application

Indications d'application: Approved: ELISA, ICC, IHC, IHC-Fr, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid freeze-thaw cycles.

Détails sur PEDF

Antigène: PEDF (SERPINF1) (serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))

Autre désignation: SERPINF1 / PEDF

Sujet: Name/Gene ID: SERPINF1

Synonyms: SERPINF1, EPC-1, OI12, PEDF, Serpin F1, PIG35, OI6

ID gène: 5176

UniProt: P36955