ACP1 Protein (AA 1-158) (His tag)

N° du produit ABIN666750

Cette protéine Recombinant ACP1 est exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour ACP1 Protein (AA 1-158) (His tag) (ABIN666750)

Antigène: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Type de proteíne: Recombinant

Activité biologique: Active

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

Pureté: > 95 %

Détail du produit

Attributs du protein: AA 1-158

Purification/Conjugué: Cette ACP1 protéine est marqué à la His tag.

Séquence: MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

Attributs du produit: Acid phosphatase 1,1-158aa, Human, His-tagged, Recombinant, E.coli

Purification: > 95% by SDS-PAGE

niveau d'endotoxine: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Specific activity is > 15,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM MES buffer ( pH 6.0) containing 0.1 mM PMSF, 2 mM EDTA, 10 % glycerol

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: ACP1 (Acid Phosphatase 1, Soluble (ACP1))

Autre désignation: LMW-PTP/ACP1

Sujet: Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Poids moléculaire: 20.1 kDa (178aa) confirmed by MALDI-TOF

NCBI Accession: NP_009030