HMGCL Protein (AA 28-325) (His tag)

N° du produit ABIN667639

Protéine Recombinant HMGCL exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour HMGCL Protein (AA 28-325) (His tag) (ABIN667639)

Antigène: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 85 %

Détail du produit

Attributs du protein: AA 28-325

Purification/Conjugué: Cette HMGCL protéine est marqué à la His tag.

Séquence: MGSSHHHHHH SSGLVPRGSH MGSHMTLPKR VKIVEVGPRD GLQNEKNIVS TPVKIKLIDM LSEAGLSVIE TTSFVSPKWV PQMGDHTEVL KGIQKFPGIN YPVLTPNLKG FEAAVAAGAK EVVIFGAASE LFTKKNINCS IEESFQRFDA ILKAAQSANI SVRGYVSCAL GCPYEGKISP AKVAEVTKKF YSMGCYEISL GDTIGVGTPG IMKDMLSAVM QEVPLAALAV HCHDTYGQAL ANTLMALQMG VSVVDSSVAG LGGCPYAQGA SGNLATEDLV YMLEGLGIHT GVNLQKLLEA GNFICQALNR KTSSKVAQAT CKL

Attributs du produit: HMGCL, 28-325aa , Human, His tag, E.coli

Purification: > 85% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 0.4M urea

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: HMGCL (3-Hydroxymethyl-3-Methylglutaryl-CoA Lyase (HMGCL))

Autre désignation: HMGCL

Sujet: HMGCL, also known as hydroxymethylglutaryl-CoA lyase, is a mitochondrial matrix protein that belongs to the HMG-CoA lyase family. It exists as a homodimer and participates in leucine catabolism and ketogenesis, the hepatic synthesis of ketone bodies that, during fasting, provide a major source of energy for heart, brain and kidney. More specifically, it catalyzes the final step of these processes, the cleavage of 3-hydroxy-3-methylglutaryl-CoA to acetoacetic acid and acetyl-CoA. Recombinant human HMGCL protein, fused to His-tag at N-terminus, was expressed in E. coli.

Poids moléculaire: 34.2 kDa (323aa)

NCBI Accession: NP_000182