Nanog Protein (AA 1-154) (His tag)

N° du produit ABIN667930

Protéine Recombinant Nanog exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour Nanog Protein (AA 1-154) (His tag) (ABIN667930)

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 90 %

Détail du produit

Attributs du protein: AA 1-154

Purification/Conjugué: Cette Nanog protéine est marqué à la His tag.

Séquence: MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQ

Attributs du produit: NANOG, 1-154aa, Human, His tag, E.coli

Purification: > 90% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 1 mM DTT

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: Nanog (NANOG) (Nanog Homeobox (NANOG))

Autre désignation: Nanog

Sujet: NANOG, also known as nanog homeobox, is a member of the homeobox family of DNA binding transcription factors that has been shown to maintain pluripotency of embryonic stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is down-regulated, cell differentiation can proceed. Recombinant human NANOG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Poids moléculaire: 19.6 kDa (174aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

NCBI Accession: NP_079141

Pathways: Stem Cell Maintenance