PAPSS1 Protein (AA 24-624) (His tag)

N° du produit ABIN668017

Protéine Recombinant PAPSS1 exprimée dans Escherichia coli (E. coli).

Aperçu rapide pour PAPSS1 Protein (AA 24-624) (His tag) (ABIN668017)

Antigène: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Type de proteíne: Recombinant

Origine: Humain

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Pureté: > 85 %

Détail du produit

Attributs du protein: AA 24-624

Purification/Conjugué: Cette PAPSS1 protéine est marqué à la His tag.

Séquence: RATNV TYQAHHVSRN KRGQVVGTRG GFRGCTVWLT GLSGAGKTTV SMALEEYLVC HGIPCYTLDG DNIRQGLNKN LGFSPEDREE NVRRIAEVAK LFADAGLVCI TSFISPYTQD RNNARQIHEG ASLPFFEVFV DAPLHVCEQR DVKGLYKKAR AGEIKGFTGI DSEYEKPEAP ELVLKTDSCD VNDCVQQVVE LLQERDIVPV DASYEVKELY VPENKLHLAK TDAETLPALK INKVDMQWVQ VLAEGWATPL NGFMREREYL QCLHFDCLLD GGVINLSVPI VLTATHEDKE RLDGCTAFAL MYEGRRVAIL RNPEFFEHRK EERCARQWGT TCKNHPYIKM VMEQGDWLIG GDLQVLDRVY WNDGLDQYRL TPTELKQKFK DMNADAVFAF QLRNPVHNGH ALLMQDTHKQ LLERGYRRPV LLLHPLGGWT KDDDVPLMWR MKQHAAVLEE GVLNPETTVV AIFPSPMMYA GPTEVQWHCR ARMVAGANFY IVGRDPAGMP HPETGKDLYE PSHGAKVLTM APGLITLEIV PFRVAAYNKK KKRMDYYDSE HHEDFEFISG TRMRKLAREG QKPPEGFMAP KAWTVLTEYY KSLEKA

Attributs du produit: PAPSS1, 24-624aa, Human, His tag, E.coli

Purification: > 85% by SDS-PAGE

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl

Conseil sur la manipulation: Avoid repeated freezing and thawing cycles

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Détail du antigène

Antigène: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Autre désignation: PAPSS1

Sujet: PAPSS1, also known as bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway, yielding 3'-phosphoadenylylsulfate (PAPS). PAPSS1 is also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells. Recombinant human PAPSS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Poids moléculaire: 70.9 kDa (626aa)

NCBI Accession: NP_005434

Pathways: Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process