GFP anticorps (FITC)

N° du produit ABIN100088

N° du produit (Fournisseur): 600-102-215

L’anticorps Chèvre Polyclonal anti-GFP a été validé pour WB, IHC, FM, DB et FLISA. Il convient pour détecter GFP dans des échantillons de Aequorea victoria. Il y a 8+ publications disponibles.

Aperçu rapide pour GFP anticorps (FITC) (ABIN100088)

Antigène: GFP (Green Fluorescent Protein (GFP))

Reactivité: Aequorea victoria

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GFP est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Fluorescence Microscopy (FM), Dot Blot (DB), FLISA

Détail du produit anti-GFP anticorps (FITC)

N° du produit (Fournisseur): 600-102-215

Fournisseur: Rockland

Fonction: GFP Antibody Fluorescein Conjugated

Réactivité croisée (Details): Minimal crossreactivity against Hu Ms & Rt Serum Proteins
   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria).

Attributs du produit: Synonyms: goat anti-GFP Antibody fluorescein Conjugation, FITC conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: GFP Fluorescein Conjugated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Labeling Ratio: 3.85

Information d'application

Indications d'application:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-GFP Fluorescein Conjugated Antibody has been tested by dot blot and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Western Blot Dilution: >1:10,000

FLISA Dilution: >1:20,000

IF Microscopy Dilution: 1:500 - 1:2,500

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-GFP (FITC) (ABIN100088)

Perna, Alberi: "TF-ChIP Method for Tissue-Specific Gene Targets." dans: Frontiers in cellular neuroscience, Vol. 13, pp. 95, (2019) (PubMed).

Hock, Maniecka, Hruska-Plochan, Reber, Laferrière, Sahadevan M K, Ederle, Gittings, Pelkmans, Dupuis, Lashley, Ruepp, Dormann, Polymenidou: "Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function." dans: Cell reports, Vol. 24, Issue 4, pp. 987-1000.e7, (2018) (PubMed).

Kwon, Everetts, Wang, Wang, Della Croce, Xing, Yao: "Controlling Depth of Cellular Quiescence by an Rb-E2F Network Switch." dans: Cell reports, Vol. 20, Issue 13, pp. 3223-3235, (2018) (PubMed).

Fong, Mazo, Das, Goodman, Kim, ORourke, Izquierdo, Tsou: "53BP1 and USP28 mediate p53-dependent cell cycle arrest in response to centrosome loss and prolonged mitosis." dans: eLife, Vol. 5, (2016) (PubMed).

Doucet, Woo, Ruiz, Owens: "The touch dome defines an epidermal niche specialized for mechanosensory signaling." dans: Cell reports, Vol. 3, Issue 6, pp. 1759-65, (2014) (PubMed).

Wang, Tay, Soni, Perumal, Goll, Macaluso, Asara, Amack, Tsou: "CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base." dans: Nature cell biology, Vol. 15, Issue 6, pp. 591-601, (2013) (PubMed).

Guo, Maeda, Ma, Delgado, Sohn, Miers, Ko, Bannerman, Xu, Wang, Zhou, Takebayashi, Pleasure: "Macroglial plasticity and the origins of reactive astroglia in experimental autoimmune encephalomyelitis." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 33, pp. 11914-28, (2011) (PubMed).

Niederkofler, Baeriswyl, Ott, Stoeckli: "Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance." dans: Development (Cambridge, England), Vol. 137, Issue 3, pp. 427-35, (2010) (PubMed).

Détails sur GFP

Antigène: GFP (Green Fluorescent Protein (GFP))

Autre désignation: GFP

Sujet: Background: GFP Fluorescein Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

UniProt: P42212