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Hub1 anticorps

Hub1 Reactivité: Saccharomyces cerevisiae WB, ELISA, IP, IHC Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN100576
  • Antigène
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    Reactivité
    Saccharomyces cerevisiae
    Hôte
    Lapin
    Clonalité
    Polyclonal
    Application
    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Immunogène
    This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
    Immunogentype:Recombinant
    Isotype
    IgG
  • Indications d'application
    This purified polyclonal antibody reacts with yeast Hub1 by western blot and ELISA. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.   This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or conjugates).  Other intrinsic bands are readily detectable at lower dilutions. For immunoblotting a 9.7 kDa band corresponding to yeast Hub1 is detected.  Most yeast cell lysates can be used as a positive control without induction or stimulation. 
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    5.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
  • Antigène
    Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
    Autre désignation
    Hub1
    Sujet
    Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin.  Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12.  Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs).  These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other.  In contrast to UBLs, UDPs are not conjugated to other proteins.  Hub1 (''Homologous to UBiquitin'') may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation.
    Synonyms: Homologous to UBiquitin antibody, Hub1 protein antibody, HUB1 target protein 1 antibody, Hub1p antibody, Ubiquitin like protein modifier antibody
    ID gène
    855767, 6324360
    UniProt
    Q6Q546
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