CD16 anticorps (PerCP)
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- Antigène Voir toutes CD16 Anticorps
- CD16
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Reactivité
- Humain, Non-Human Primate
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp CD16 est conjugé à/à la PerCP
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Application
- Flow Cytometry (FACS)
- Specificité
- The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils). Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
- Réactivité croisée (Details)
- Human, Non-Human Primates
- Purification
- Purified antibody is conjugated with activated Peridinin-Chlorophyll Protein (PerCP) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogène
- Human neutrophils
- Clone
- 3G8
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product CD16 Anticorps primaire
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- Indications d'application
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
- Commentaires
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The purified antibody is conjugated with Peridinin-chlorophyll-protein complex (PerCP) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
- Restrictions
- For Research Use only
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- Reconstitution
- No reconstitution is necessary.
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze.
Avoid prolonged exposure to light. - Stock
- 4 °C
- Stockage commentaire
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, (2009) (PubMed).
: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." dans: Immunology, Vol. 124, Issue 2, pp. 215-22, (2008) (PubMed).
: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." dans: Cancer research, Vol. 68, Issue 4, pp. 976-80, (2008) (PubMed).
: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." dans: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, (2007) (PubMed).
: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." dans: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, (2005) (PubMed).
: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." dans: Blood, Vol. 91, Issue 7, pp. 2369-80, (1998) (PubMed).
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The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, (2009) (PubMed).
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- Antigène
- CD16
- Autre désignation
- CD16 (CD16 Produits)
- Sujet
- CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.,FcgammaRIII, IGFR3, FCRIII
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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