SLC17A7 anticorps (AA 493-560)

N° du produit ABIN1027710

Cet anticorps anti-SLC17A7 Monoclonal Souris (Clone S28-9) (ABIN1027710) détecte spécifiquement SLC17A7 dans WB, IHC et mIHC. L’anticorps est réactif avec des échantillons de Rat. Validé indépendamment pour une utilisation en Multiplex Immunohistochemistry.

Aperçu rapide pour SLC17A7 anticorps (AA 493-560) (ABIN1027710)

Antigène: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp SLC17A7 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Multiplex Immunohistochemistry (mIHC)

Clone: S28-9

Détail du produit anti-SLC17A7 anticorps

Épitope: AA 493-560

Specificité: Detects ~52 kDa. No cross-reactivity against VGlut2.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 493-560 (cytoplasmic C-terminus) of rat VGlut1

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1027710 was sufficient for detection of VGLut1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Independent Validation (mIHC)

Validation #104338 (Multiplex Immunohistochemistry)

by: Akoya Biosciences

No.: #104338

Date: 20.04.2021

Antigène: SLC17A7

Numéro du lot: 1205

Application validée: Multiplex Immunohistochemistry

Contrôle positif:

Fresh frozen mouse olfactory bulb

Contrôle négative:

Unlabeled control (mouse fresh frozen)

Conclusion:

Passed. The anti-SLC17A7 antibody ABIN1027710 labels areas of high glutamatergic afferent input throughout the murine olfactory bulb with a concentration in the internal plexiform and granule cell layer.

Validation Images

Murine fresh frozen coronal olfactory bulb section (Thickness = 5 µm) labeled with anti-SLC17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550). Labeling is present throughout olfactory bulb layers with a concentration in the internal plexiform and granule cell layers. Map2 and PSD-95/DLG4 were labeled with ABIN125739 (blue; bound to fluorophore ATTO 550) and ABIN361694 (red; bound to fluorophore ATTO 550).

FFPE normal human cortex tissue section labeled with anti-SL17A7 antibody ABIN1027710 (green; bound to fluorophore ATTO 550) after EDTA antigen retrieval. MAP2 and dopamine transporter were labeledd with anti-MAP2 antibody ABIN125739 (red; bound to fluorophore ATTO 550) and anti-dopamine receptor antibody (blue; bound to fluorophore AF647).

Protocole

Anticorps primaire: ABIN1027710

Full Protocol:

• Protocol details are described in the Akoya Biosciences CODEX® User Manual (see https://www.akoyabio.com/wp-content/uploads/2021/01/CODEX-User-Manual.pdf).
• Tissue preparation as outlined in the Akoya Biosciences CODEX® User Manual fresh-frozen tissue protocol.
• Conjugation of the anti-SLC17A7 antibody ABIN1027710 to an oligo barcode used to bind oligo-conjugated fluorophore ATTO 550.

Notes:

• No signal was detected in unlabeled specimens.

• Specific staining of Cortactin was also observed with human FFPE cortical tissue sections with both citrate antigen retrieval and EDTA antigen retrieval.

• Optimal staining and signal to noise ratios were obtained if tissue was pre-treated for autofluorescence removal (see https://www.akoyabio.com/wp-content/uploads/2020/07/Customer-Demonstrated-Protocol-Autofluorescence-Quenching-Mar2020.pdf).

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur SLC17A7

Antigène: SLC17A7 (Solute Carrier Family 17 (Vesicular Glutamate Transporter), Member 7 (SLC17A7))

Autre désignation: VGLUT1

Sujet: VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate (1). Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate (2).

ID gène: 116638

NCBI Accession: NP_446311

UniProt: Q62634