GABBR1 anticorps (AA 873-977)

N° du produit ABIN1027717

Cet anticorps Souris Monoclonal détecte spécifiquement GABBR1 dans WB, IF et ICC. Il présente une réactivité envers Rat.

Aperçu rapide pour GABBR1 anticorps (AA 873-977) (ABIN1027717)

Antigène: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GABBR1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: S93A-49

Détail du produit anti-GABBR1 anticorps

Épitope: AA 873-977

Specificité: Detects ~115 kDa. No cross-reactivity against GABA(B)R2.

Réactivité croisée: Humain, Souris, Rat

Purification: Protein G Purified

Immunogène: Fusion protein amino acids 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1

Isotype: IgG1

Information d'application

Indications d'application:

• WB (1:1000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

1 μg/ml of ABIN1027717 was sufficient for detection of GABA(B)R1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: -20°C

Détails sur GABBR1

Antigène: GABBR1 (gamma-aminobutyric Acid (GABA) B Receptor, 1 (GABBR1))

Autre désignation: GABA B Receptor 1

Sujet: GABA (γ-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B) and GABA(C) receptor. The ionotropic GABA(A) and GABA(C) receptors are ligand-gated ion channels that produce fast inhibitory synaptic transmission. In contrast, the metabotropic GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission (1). Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 where GABA(B)R1 binds the ligand and GABA(B)R2 is the primary G protein contact site (2). Two isoforms of GABA(B)R1 have been cloned: GABA(B)R1a is a 130 kD protein and GABA(B)R1b is a 95 kD protein (3). G proteins subsequently inhibit adenyl cylase activity and modulate inositol phospholipid hydrolysis. GABA(B) receptors have both pre- and postsynaptic inhibitions: presynaptic GABA(B) receptors inhibit neurotransmitter release through suppression of high threshold calcium channels, while postsynaptic GABA(B) receptors inhibit through coupled activation of inwardly rectifying potassium channels. In addition to synaptic inhibition, GABA(B) receptors may also be involved in hippocampal long-term potentiation, slow wave sleep and muscle relaxation (1).

ID gène: 81657

NCBI Accession: NP_112290

UniProt: Q9Z0U4

Pathways: Positive Regulation of Peptide Hormone Secretion, cAMP Metabolic Process