SARS-CoV-2 Spike anticorps (C-Term)

N° du produit ABIN1030641

Cet anticorps anti-SARS-CoV-2 Spike est un anticorps Lapin Polyclonal détectant SARS-CoV-2 Spike dans ELISA, WB, IF et IHC. Adapté pour SARS Coronavirus-2 (SARS-CoV-2) et SARS Coronavirus (SARS-CoV). Ce Primary Antibody a été cité dans 11+ publications.

Aperçu rapide pour SARS-CoV-2 Spike anticorps (C-Term) (ABIN1030641)

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Reactivité: SARS Coronavirus-2 (SARS-CoV-2), SARS Coronavirus (SARS-CoV)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SARS-CoV-2 Spike est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Détail du produit anti-SARS-CoV-2 Spike anticorps

Épitope: C-Term

Homologie: Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)

Purification: Affinity chromatography purified via peptide column

Immunogène: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Spike antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike glycoprotein.
The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein.

Isotype: IgG

Information d'application

Indications d'application: WB: 1 μg/mL; IF: 1 μg/mL. IHC: 0.2 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: The antibody is supplied in PBS containing 0.02% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Stock: 4 °C/-20 °C

Stockage commentaire: Antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Date de péremption: 12 months

Références pour anticorps anti-SARS-CoV-2 Spike (ABIN1030641)

Wardhana, Kuntaman, Utomo, Aryananda, Rifdah, Wafa, Shahnaz, Ningrum, Cininta, Ariani, Van Lith, Dachlan: "Evidence of Placental Villous Inflammation and Apoptosis in Third-Trimester Symptomatic SARS-CoV-2 Maternal Infection." dans: Yonsei medical journal, Vol. 65, Issue 4, pp. 202-209, (2024) (PubMed).

Basu, Penumutchu, Nguyen, Mbonye, Tolbert, Karn, Komar, Mazumder: "A Structurally Conserved RNA Element within SARS-CoV-2 ORF1a RNA and S mRNA Regulates Translation in Response to Viral S Protein-Induced Signaling in Human Lung Cells." dans: Journal of virology, Vol. 96, Issue 2, pp. e0167821, (2022) (PubMed).

El Jamal, Pujadas, Ramos, Bryce, Grimes, Amanat, Tsankova, Mussa, Olson, Salem, Miorin, Aydillo, Schotsaert, Albrecht, Liu, Marjanovic, Francoeur, Sebra, Sealfon, García-Sastre, Fowkes, Cordon-Cardo et al.: "Tissue-Based SARS-Cov-2 Detection in Fatal COVID-19 Infections: Sustained Direct Viral-Induced Damage is Not Necessary to Drive Disease Progression. ..." dans: Human pathology, (2021) (PubMed).

Garifullina, Shen: "High-throughput fabrication of high aspect ratio Ag/Al nanopillars for optical detection of biomarkers." dans: Journal of materials chemistry. B, (2021) (PubMed).

Magro, Mulvey, Berlin, Nuovo, Salvatore, Harp, Baxter-Stoltzfus, Laurence: "Complement associated microvascular injury and thrombosis in the pathogenesis of severe COVID-19 infection: A report of five cases." dans: Translational research : the journal of laboratory and clinical medicine, Vol. 220, pp. 1-13, (2020) (PubMed).

Mulvey, Magro, Ma, Nuovo, Baergen: "Analysis of complement deposition and viral RNA in placentas of COVID-19 patients." dans: Annals of diagnostic pathology, Vol. 46, pp. 151530, (2020) (PubMed).

Nuovo, Tili, Suster, Matys, Hupp, Magro et al.: "Strong homology between SARS-CoV-2 envelope protein and a Mycobacterium sp. antigen allows rapid diagnosis of Mycobacterial infections and may provide specific anti-SARS-CoV-2 immunity via the BCG ..." dans: Annals of diagnostic pathology, Vol. 48, pp. 151600, (2020) (PubMed).

Nuovo, Magro, Mikhail: "Cytologic and molecular correlates of SARS-CoV-2 infection of the nasopharynx." dans: Annals of diagnostic pathology, Vol. 48, pp. 151565, (2020) (PubMed).

Nuovo, Magro, Shaffer, Awad, Suster, Mikhail, He, Michaille, Liechty, Tili: "Endothelial cell damage is the central part of COVID-19 and a mouse model induced by injection of the S1 subunit of the spike protein." dans: Annals of diagnostic pathology, Vol. 51, pp. 151682, (2020) (PubMed).

Ventura, Cennamo, Minopoli, Campanile, Censi, Terracciano, Portella, Velotta: "Colorimetric Test for Fast Detection of SARS-CoV-2 in Nasal and Throat Swabs." dans: ACS sensors, Vol. 5, Issue 10, pp. 3043-3048, (2020) (PubMed).

Funari, Chu, Shen: "Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip." dans: Biosensors & bioelectronics, Vol. 169, pp. 112578, (2020) (PubMed).

Détails sur SARS-CoV-2 Spike

Antigène: SARS-CoV-2 Spike (SARS-CoV-2 S)

Autre désignation: SARS-CoV-2 Spike

Classe de substances: Viral Protein

Sujet: Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak. The structure of 2019-nCoV consists of the following: a Spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. Coronavirus invades cells through Spike (S) glycoproteins, a class I fusion protein. It is the major viral surface protein that coronavirus uses to bind to the human cell surface receptor. It also mediates the fusion of host and viral cell membrane, allowing the virus to enter human cells and begin infection. The spike protein is the major target for neutralizing antibodies and vaccine development. The protein modeling suggests that there is strong interaction between Spike protein receptor-binding domain and its host receptor angiotensin-converting enzyme 2 (ACE2), which regulate both the cross-species and human-to-human transmissions of COVID-19. The recent study has shown that the SARS-CoV-2 spike protein binds ACE2 with higher affinity than SARS-CoV spike protein .

NCBI Accession: QHD43416