LIM Domain Binding 1 Protein anticorps (C-Term)

N° du produit ABIN103458

N° du produit (Fournisseur): 600-401-350

Cet anticorps anti-LIM Domain Binding 1 Protein est un anticorps Lapin Polyclonal détectant LIM Domain Binding 1 Protein dans WB, ELISA et ChIP. Adapté pour Humain.

Aperçu rapide pour LIM Domain Binding 1 Protein anticorps (C-Term) (ABIN103458)

Antigène: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIM Domain Binding 1 Protein est non-conjugé

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Détail du produit anti-LIM Domain Binding 1 Protein anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-350

Fournisseur: Rockland

Fonction: LDB1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against the mouse LDB1protein.

Attributs du produit: Synonyms: rabbit anti-LDB1 antibody, rabbit anti-LDB1/CLIM2 antibody, rabbit anti-CLIM2 antibody, LDB-1, CLIM-2, LIM domain-binding protein 1, Carboxyl-terminal LIM domain-binding protein 2, LIM domain-binding factor CLIM2, Nuclear LIM interactor

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region of mouse LDB1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This affinity purified antibody has been tested for use in ELISA, western blot and CHIP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 43 kDa in size corresponding to LDB1 by western blotting in the appropriate cell lysate or extract. This antibody has been used in a ChIP assay using murine erythroleukemia (MEL) cells. The test sequence was the upstream enhancer of the GATA-1 gene, a putative LDB1 binding region as suggested by Orkin et al.   Anti-LDB1 was used successfully in ChIP assays to precipitate a roughly 4-fold enrichment at the GATA1-HS1 enhancer element in DMSO-induced murine erythroleukemia cells.  We suggest using 20 μg for 10E8 cells for ChIP.

ChIP Dilution: User Optimized

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:425,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur LIM Domain Binding 1 Protein

Antigène: LIM Domain Binding 1 Protein (LDB1) (LIM Domain Binding 1 (LDB1))

Autre désignation: Ldb1

Sujet: Background: LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

ID gène: 16825, 6754520

UniProt: P70662

Pathways: Stem Cell Maintenance, Chromatin Binding