CD43 anticorps

N° du produit ABIN1042482

Détail du produit anti-CD43 anticorps

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: Clone MT1 produces mouse IgG1 immunoglobulins reactive with a 95-115 kD highly sialated glycoprotein. This antibody is used to identify B cell lines and myeloma cells. It may be used in the diagnosis of chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.

Attributs du produit: CD markers

Purification: Purified

Clone: MT1

Isotype: IgG1

Information d'application

Indications d'application: The antibody is suitable for flow cytometry for analysis of blood and bone marrow samples or in immunohistochemistry using cytospots, paraffin or frozen tissue sections. Optimal antibody dilution should be determined by titration.

Commentaires:

MT1 is a monoclonal antibody derived by fusion of X63 mouse myeloma cells with spleen cells from of Balb/c mice, intraperitoneally immunized with human monocytes isolated by centrifugal elutriation. Immunizations were carried out at least twice at 2-week intervals with: A) cell suspensions of a lymph node involved by Hodgkin's disease, B) cell suspensions of a lymph node involved by Bchronic lymphatic leukemia, C) cells of Hodgkin's cell line DEV, respectively. No adjuvants were used (3).

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.09% sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Références pour anticorps anti-CD43 (ABIN1042482)

Manjunath, Correa, Ardman, Ardman: "Negative regulation of T-cell adhesion and activation by CD43." dans: Nature, Vol. 377, Issue 6549, pp. 535-8, (1995) (PubMed).

Schmid, Hediger, Brossmer, Collins, Haupt, Marti, Offner, Schaller, Takagaki, Walsh: "Amino acid sequence of human plasma galactoglycoprotein: identity with the extracellular region of CD43 (sialophorin)." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 2, pp. 663-7, (1992) (PubMed).

Poppema, Hollema, Visser, Vos: "Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections." dans: The American journal of pathology, Vol. 127, Issue 3, pp. 418-29, (1987) (PubMed).

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: CD43 (SPN Produits)

Synonymes: anticorps CD43, anticorps GALGP, anticorps GPL115, anticorps LSN, anticorps A630014B01Rik, anticorps Cd43, anticorps Galgp, anticorps Ly-48, anticorps Ly48, anticorps Lsn, anticorps Lsn1, anticorps gpL115, anticorps sialophorin, anticorps SPN, anticorps Spn

Sujet: CD43 antigen is expressed on all T cells, NK cells, myeloid cells and monocytes as well as on CD5 positive mature B cells. It is also present on all immature hematopoietic cells in the bone marrow. The antigen is deficient in patients with Wiskott-Aldrich syndrome. A soluble form of CD43 is present in human serum.

Pathways: Regulation of Leukocyte Mediated Immunity