Vimentin anticorps (FITC)

N° du produit ABIN1042493

Il existe 5+ publications pour ce produit. L’anticorps anti-Vimentin Monoclonal Souris est utilisé pour la détection de Vimentin dans des échantillons de Différentes espèces. Il a été validé pour FACS, ICC, IHC (fro), IHC (p) et WB.

Aperçu rapide pour Vimentin anticorps (FITC) (ABIN1042493)

Antigène: Vimentin (VIM)

Reactivité: Différentes espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: RV202

Détail du produit anti-Vimentin anticorps (FITC)

Fonction: Mouse anti Vimentin, conjugated with FITC

Specificité: RV202-FITC reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-FITC is suitable for immunocytochemistry on cell cultures and cytospins, immunohistochemistry on frozen tissues, as well as for flow cytometry. Optimal antibody dilution should be determined by titration, recommended dilution is 1:10.

Attributs du produit: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Immunogène: RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 1 mL FITC-conjugated vimentin monoclonal antibody in PBS containing 0,1 % BSA, 0.09 % sodium azide. Approximately 100 tests.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Références pour anticorps anti-Vimentin (FITC) (ABIN1042493)

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." dans: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." dans: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis