Vimentin anticorps (HRP)

N° du produit ABIN1042494

Cité dans 5 publications. Cet anticorps anti-Vimentin Monoclonal Souris conjugué à HRP (Clone RV202) (ABIN1042494) détecte spécifiquement Vimentin dans FACS, ICC, IHC (fro), IHC (p) et WB. L’anticorps est réactif avec des échantillons de Différentes espèces.

Aperçu rapide pour Vimentin anticorps (HRP) (ABIN1042494)

Antigène: Vimentin (VIM)

Reactivité: Différentes espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est conjugé à/à la HRP

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Clone: RV202

Détail du produit anti-Vimentin anticorps (HRP)

Fonction: Mouse anti Vimentin, conjugated with HRP

Specificité: RV202-HRP reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchyme derived tumors e.g. lymphoma, sarcoma and melanoma. RV202-HRP is suitable for immunoblotting, immunocytochemistry on cell cultures and cytospins, as well as immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration, recommended dilution is 1:10.

Attributs du produit: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Immunogène: RV202 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Each vial contains 1 mL sterile filtered HRP-conjugated vimentin monoclonal antibody in PBS containing 0.1 % BSA. Approximately 100 tests.

Stock: 4 °C,-20 °C

Stockage commentaire: The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Références pour anticorps anti-Vimentin (HRP) (ABIN1042494)

Raats, Pieper, Vree Egberts, Verrijp, Ramaekers, Bloemendal: "Assembly of amino-terminally deleted desmin in vimentin-free cells." dans: The Journal of cell biology, Vol. 111, Issue 5 Pt 1, pp. 1971-85, (1990) (PubMed).

Ramaekers, van Niekerk, Poels, Schaafsma, Huijsmans, Robben, Schaart, Vooijs: "Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas." dans: The American journal of pathology, Vol. 136, Issue 3, pp. 641-55, (1990) (PubMed).

Pieper, Schaart, Krimpenfort, Henderik, Moshage, van de Kemp, Ramaekers, Berns, Bloemendal: "Transgenic expression of the muscle-specific intermediate filament protein desmin in nonmuscle cells." dans: The Journal of cell biology, Vol. 108, Issue 3, pp. 1009-24, (1989) (PubMed).

Viebahn, Lane, Ramaekers: "Keratin and vimentin expression in early organogenesis of the rabbit embryo." dans: Cell and tissue research, Vol. 253, Issue 3, pp. 553-62, (1988) (PubMed).

Ramaekers, Huysmans, Schaart, Moesker, Vooijs: "Tissue distribution of keratin 7 as monitored by a monoclonal antibody." dans: Experimental cell research, Vol. 170, Issue 1, pp. 235-49, (1987) (PubMed).

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: Vimentin

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis