gamma-Tubulin GCP2 (AA 2-194) anticorps

N° du produit ABIN1043030

L’anticorps Souris Monoclonal anti- a été validé pour ICC, IHC (p), IP et WB. Il convient pour détecter dans des échantillons de Humain, Souris, Porc et Rat. Il y a 2+ publications disponibles.

Aperçu rapide pour gamma-Tubulin GCP2 (AA 2-194) anticorps (ABIN1043030)

Antigène: gamma-Tubulin GCP2

Reactivité: Humain, Souris, Porc, Rat

Hôte: Souris

Clonalité: Monoclonal

Application: Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)

Clone: GCP2-01

Détail du produit

Épitope: AA 2-194

Fonction: Anti-gamma-Tubulin GCP2 Purified

Specificité: The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.

Réactivité croisée (Details): Human, Porcine, Mouse, Rat

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: GST-fusion protein containing amino acids 2-194 of mouse GCP2

Isotype: IgG2b kappa

Information d'application

Indications d'application: Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Référence

Dráberová, DAgostino, Caracciolo, Sládková, Sulimenko, Sulimenko, Sobol, Maounis, Tzelepis, Mahera, K?en, Legido, Giordano, Mörk, Hozák, Dráber, Katsetos: "Overexpression and Nucleolar Localization of ?-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma." dans: Journal of neuropathology and experimental neurology, Vol. 74, Issue 7, pp. 723-42, (2015) (PubMed).

Ho?ejší, Vinopal, Sládková, Dráberová, Sulimenko, Sulimenko, Vosecká, Philimonenko, Hozák, Katsetos, Dráber: "Nuclear ?-tubulin associates with nucleoli and interacts with tumor suppressor protein C53." dans: Journal of cellular physiology, Vol. 227, Issue 1, pp. 367-82, (2011) (PubMed).

Détail du antigène

Antigène: gamma-Tubulin GCP2

Sujet: Tubulin gamma complex associated protein 2,Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.,GCP-2, GTCC2, GTC2

ID gène: 10844

UniProt: Q9BSJ2