B-Phycoerythrin anticorps

N° du produit ABIN1043760

N° du produit (Fournisseur): 200-401-099

Cet anticorps anti-B-Phycoerythrin est un anticorps Lapin Polyclonal détectant B-Phycoerythrin dans WB, ELISA, IHC et DB. Adapté pour Red algae (Porphyridium cruentum). Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour B-Phycoerythrin anticorps (ABIN1043760)

Antigène: B-Phycoerythrin (Phycoerythrin, B-)

Reactivité: Red algae (Porphyridium cruentum)

Hôte: Lapin

Clonalité: Polyclonal

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-B-Phycoerythrin anticorps

N° du produit (Fournisseur): 200-401-099

Fournisseur: Rockland

Fonction: B-Phycoerythrin Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].

Attributs du produit: Synonyms: rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin

Purification: Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Phycoerythrin [Porphyridium creuentum]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-B-Phycoerythrin antibody has been tested by ELISA and dot blot and is suitable for western blot and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:20,000 - 1:100,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-B-Phycoerythrin (ABIN1043760)

Firl, Degn, Padera, Carroll: "Capturing change in clonal composition amongst single mouse germinal centers." dans: eLife, Vol. 7, (2018) (PubMed).

Détails sur B-Phycoerythrin

Antigène: B-Phycoerythrin (Phycoerythrin, B-)

Autre désignation: B-Phycoerythrin

Sujet: Background: Anti-B-Phycoerythrin Antibody detects the B-PE phycoerythrin protein. Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, and red algae. Phycoerythrin is organized in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. R-Phycoerythrin and B-Phycoerythrin are among the brightest biological fluorescent dyes and are commonly used as a fluorescent dye in many assays and is frequently conjugated to antibodies as a reporter molecule. The spectral properties of B-Phycoerythrin are absorption at 545 nm and emission at 572 nm.

UniProt: P11392