P35 anticorps

N° du produit ABIN1043768

N° du produit (Fournisseur): 200-401-c12

L’anticorps Lapin Polyclonal anti- a été validé pour WB et ELISA. Il convient pour détecter dans des échantillons de Borrelia burgdorferi. Il y a 1 publication disponible.

Aperçu rapide pour P35 anticorps (ABIN1043768)

Antigène: P35

Reactivité: Borrelia burgdorferi

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Détail du produit

N° du produit (Fournisseur): 200-401-c12

Fournisseur: Rockland

Fonction: p35 Antibody

Réactivité croisée (Details): This antibody is specific for Borrelia burgdorferi p35 protein.

Attributs du produit: Synonyms: rabbit anti-p35 Antibody, bba64, Borrelia burgdorferi p35

Purification: This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.

Immunogène:

Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi p35 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This protein-A purified antibody has been tested for use in Western blotting and ELISA. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 27.1 kDa in size corresponding to Borrelia burgdorferi OspA protein by Western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: >1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Gautam, Hathaway, McClain, Ramesh, Ramamoorthy: "Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter." dans: Microbiology (Reading, England), Vol. 154, Issue Pt 1, pp. 275-85, (2008) (PubMed).

Détail du antigène

Antigène: P35

Sujet: Background: The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34 °C but not 24 °C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH , being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35- kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease.

ID gène: 1194146

NCBI Accession: WP_010256558

UniProt: O50687