IL-25 anticorps (Biotin)

N° du produit ABIN1043795

N° du produit (Fournisseur): 209-306-c58

L’anticorps Souris Monoclonal anti-IL-25 a été validé pour WB, ELISA et FACS. Il convient pour détecter IL-25 dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour IL-25 anticorps (Biotin) (ABIN1043795)

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-25 est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Clone: 11K8B1

Détail du produit anti-IL-25 anticorps (Biotin)

N° du produit (Fournisseur): 209-306-c58

Fournisseur: Rockland

Fonction: IL-17E Biotin Conjugated Antibody

Réactivité croisée (Details): This antibody is specific for human IL-17E protein.

Attributs du produit: Synonyms: mouse Anti-IL-17E Biotin Conjugated Antibody, mouse anti-IL-25 biotin conjugated antibody, Interleukin 25, IL-25, Interleukin 17E, IL-17E

Purification: This product was purified from concentrated tissue culture supernate by Protein G chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Anti-IL-17E (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17E produced in E.coli followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG1

Labeling Ratio: 10-20

Information d'application

Indications d'application:

Flow Cytometry Dilution: 1 μg/mL

Application Note: This purified antibody has been tested for use in Flow Cytometry and western blotting. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.5 μg/mL

ELISA Dilution: 1:10,000 - 1:50,000

Other: User optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IL-25 (Biotin) (ABIN1043795)

Conti, Shen, Nayyar, Stocum, Sun, Lindemann, Ho, Hai, Yu, Jung, Filler, Masso-Welch, Edgerton, Gaffen: "Th17 cells and IL-17 receptor signaling are essential for mucosal host defense against oral candidiasis." dans: The Journal of experimental medicine, Vol. 206, Issue 2, pp. 299-311, (2009) (PubMed).

Détails sur IL-25

Antigène: IL-25 (IL25) (Interleukin 25 (IL25))

Autre désignation: IL25

Sujet: Background: Anti-IL17E recognizes IL-17E (also known as IL25). IL-17E is produced and secreted by intra-epithelial lymphocytes, lung epithelial cells, alveolar macrophages, eosinophils, basophils, NKT cells, mast cells and cells of the gastro-intestinal tract and uterus. IL-17E stimulates the production of Th2-type cytokines. IL-17E limits Th17 cell development by inducing the expression of IL-13 by dendritic cells or by inhibiting IL-23 production by macrophages. Induces activation of NF-kappa-B and stimulates production of the proinflammatory chemokine IL-8. A number of cytokines, including IL-17E, have been implicated as oncogenes, reflecting the importance of these proteins in cell growth and survival. Anti-IL-17E cytokine antibody is ideal for investigators involved in Immunology, Signal Transduction research, Cancer and pro-inflammatory pathologies.

ID gène: 64806

NCBI Accession: NP_073626

UniProt: Q9H293