IL-27 anticorps

N° du produit ABIN1043835

N° du produit (Fournisseur): 210-501-b54

Cet anticorps anti-IL-27 est un anticorps Rat Monoclonal détectant IL-27 dans WB, ELISA, FACS et MA. Adapté pour Souris. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour IL-27 anticorps (ABIN1043835)

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IL-27 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Multiplex Assay (MA)

Clone: 3H12-F10

Détail du produit anti-IL-27 anticorps

N° du produit (Fournisseur): 210-501-b54

Fournisseur: Rockland

Fonction: Mouse IL-27/p28 Antibody

Réactivité croisée (Details): This antibody is specific for mouse and rat p28 protein.

Attributs du produit: Synonyms: rat anti-IL-27/p28 antibody, rat anti-interleukin 27a antibody, Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30

Purification: IL-27 / p28 antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-IL-27/p28 monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Information d'application

Indications d'application:

Flow Cytometry Dilution: 1 μg / ml

Application Note: IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in Flow Cytometry, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-IL-27 (ABIN1043835)

Murugaiyan, Mittal, Weiner: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, (2010) (PubMed).

Détails sur IL-27

Antigène: IL-27 (IL27) (Interleukin 27 (IL27))

Autre désignation: Il27

Sujet: Background: Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.

ID gène: 246779

NCBI Accession: NP_663611

UniProt: Q8K3I6