ADRA2C anticorps (Extracellular Domain)

N° du produit ABIN1048315

Cet anticorps Lapin Polyclonal détecte spécifiquement ADRA2C dans IHC et IHC (p). Il présente une réactivité envers Humain et Singe.

Aperçu rapide pour ADRA2C anticorps (Extracellular Domain) (ABIN1048315)

Antigène: ADRA2C (Adrenergic, alpha-2C-, Receptor (ADRA2C))

Reactivité: Humain, Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADRA2C est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ADRA2C anticorps

Épitope: Extracellular Domain

Marque: IHC-plus™

Specificité: Human ADRA2C. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Homologie: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%) Mouse, Rat, Panda, Bovine, Dog, Guinea pig (94%) Hamster (89%).

Purification: Immunoaffinity purified

Immunogène: Synthetic 18 amino acid peptide from 2nd extracellular domain of human ADRA2C. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%), Mouse, Rat, Panda, Bovine, Dog, Guinea pig (94%), Hamster (89%).

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: IHC, IHC-P (15 - 30 μg/mL)

Commentaires:

Target Species of Antibody: Human

Procédure de l'essai:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Date de péremption: 12 months

Détails sur ADRA2C

Antigène: ADRA2C (Adrenergic, alpha-2C-, Receptor (ADRA2C))

Autre désignation: ADRA2C

Sujet: Name/Gene ID: ADRA2C
Subfamily: Adrenergic
Family: GPCR

Synonyms: ADRA2C, ADRA2RL2, ADRARL2, Alpha-2C adrenergic receptor, Alpha-2c-2 adrenergic receptor, ADRA2L2, Adrenoceptor alpha 2C, Alpha-2C-adrenergic receptor, Alpha-2C-AR, Alpha-2CAR, Alpha-2C adrenoceptor, Alpha-2C adrenoreceptor, Alpha2c, ALPHA2CAR, Alpha 2c, Alpha2-AR-C4, Alpha2cii-adrenergic receptor

ID gène: 152

Pathways: EGFR Signaling Pathway, cAMP Metabolic Process